Reprogramming of T Cells from Human Peripheral Blood
نویسندگان
چکیده
منابع مشابه
Reprogramming of T cells from human peripheral blood.
Supplemental Experimental Procedures Production of virus. Cell culture and human iPS cell induction. Donor blood cells collection and isolation. Immunohistochemistry staining. qRT–PCR. Global gene expression. Bisulfite genomic sequencing. DNA fingerprinting and karyotyping. Embryoid body formation. Hematopoietic colony-forming assays. Assay for teratoma formation. Flow cytometry. Southern Blot ...
متن کاملCellular Reprogramming of Human Peripheral Blood Cells
Breakthroughs in cell fate conversion have made it possible to generate large quantities of patient-specific cells for regenerative medicine. Due to multiple advantages of peripheral blood cells over fibroblasts from skin biopsy, the use of blood mononuclear cells (MNCs) instead of skin fibroblasts will expedite reprogramming research and broaden the application of reprogramming technology. Thi...
متن کاملGene Expression Profile of Adherent Cells Derived From Human Peripheral Blood: Evidence of Mesenchymal Stem Cells
Mesenchymal stem cells (MSCs) provide a novel option in cellular therapy and tissue engineering. Recent studies indicated that it is possible to obtain MSCs from peripheral blood by attachment ability to plastic surface. To evaluate adherent cells derived from peripheral blood, their expression profile and surface markers were investigated. The results of RT-PCR indicated that these cells expre...
متن کاملOptimization of human peripheral blood mononuclear cells (PBMCs) cryopreservation
Cryopreservation is the method of choice for long term storage of human PBMCs. This study was designed to compare the different combinations of variables affecting the cryopreservation of PBMCs samples. The viability of PBMCs separated from 2×5 ml peripheral blood samples obtained from 16 healthy adult volunteers, were measured using trypan blue dye exclusion method just before freezing with ...
متن کاملReplication of human cytomegalovirus in human peripheral blood T cells.
The replication of human cytomegalovirus (HCMV) strain AD169 was studied in human peripheral blood granulocytes, monocytes-macrophages, B lymphocytes, and T lymphocytes. Progeny virus was produced in some T-cell cultures stimulated in the allogeneic mixed lymphocyte reaction and was regularly obtained when stimulated T cells were grown in the presence of interleukin 2. Replication of HCMV in th...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Cell Stem Cell
سال: 2010
ISSN: 1934-5909
DOI: 10.1016/j.stem.2010.06.004